Part:BBa_K2973006:Design
32B Toehold Switch_Enhanced Green Fluorescent Protein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch sequence was taken from Pardee et al., 2016
Source
Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.
References
Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.
Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.
Zhang, Guohong, et al. “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells.” Biochemical and Biophysical Research Communications, vol. 227, no. 3, 1996, pp. 707–711., doi:10.1006/bbrc.1996.1573.